B. Experimentation is not a stage of Discovery method.
Experiments are done based on some existing theory.
At the process of discovery first a generalized concept is taken.
After that the identification of various problems is made before reaching to the evaluation stage.
When a new discovery gets established only then the experimentation method starts to work.
A IS OMMITED
Explanation:before - is
ommited - a
after - method
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finger printing is the method of identification
Identification method o
It could be difficult to know if you were just using a random bacteria isolated from nature — especially since there are likely to be many thousands of different plasmids (1730 were present in a sequence database as of 2009). We could sequence all the DNA inside the bacteria, but that is still a lot of work ...
However this doesn't matter as much as you might think.
For example, assume we are using a plasmid that contains a marker (selectable gene) encoding resistance to ampicillin. All we need to know is that the bacteria were are transforming are not already resistant to ampicillin. This is easy to test — we just try growing the bacteria in the presence of ampicillin, if they don't then we can use our plasmid.
In practice microbiologists have domesticated strains of bacteria (a favorite is Escherichia coli — often abbreviated to E. coli) that have been studied for decades. In almost all cases you would be using one of these well characterized strains and so would not need to worry about whether there were unknown plasmids.
l ᴛʜᴇ ʙʟᴀᴄᴋʟɪsᴛ-ʙᴀsᴇᴅ ᴛᴇᴄʜɴᴏʟᴏɢʏ ɪs ᴍᴀɪɴʟʏ ɪᴍᴘʟᴇᴍᴇɴᴛᴇᴅ ʙʏ ᴍᴀɪɴᴛᴀɪɴɪɴɢ ᴀ ʙʟᴀᴄᴋʟɪsᴛ ᴡɪᴛʜ ɪᴘ ᴀᴅᴅʀᴇss ᴀɴᴅ ᴅᴏᴍᴀɪɴ ɴᴀᴍᴇ. ɪᴛ ᴄᴀɴ ʙᴇ ᴊᴜᴅɢᴇᴅ ᴛʜᴇ ᴍᴀʟɪᴄɪᴏᴜs ᴡᴇʙsɪᴛᴇ ʙʏ ᴄʜᴇᴄᴋɪɴɢ ᴡʜᴇᴛʜᴇʀ ᴛʜᴇ ᴅᴏᴍᴀɪɴ ɴᴀᴍᴇ/ɪᴘ ᴀᴅᴅʀᴇss ᴀᴘᴘᴇᴀʀs ᴏɴ ᴛʜᴇ ʙʟᴀᴄᴋʟɪsᴛ. ... ʜᴏᴡᴇᴠᴇʀ, sᴜᴄʜ ᴀᴄᴛɪᴠᴇ ᴍᴇᴛʜᴏᴅs ᴏғᴛᴇɴ ғᴀɪʟ ᴛᴏ ᴅᴇᴛᴇᴄᴛ ᴍᴀʟɪᴄɪᴏᴜs ᴡᴇʙsɪᴛᴇs ɪɴ ᴛɪᴍᴇ.
Nucleic acid–based (NA-based) detection techniques are becoming fundamental for the applied plant pathologist. Their speed, sensitivity, specificity, versatility have resulted in the use of these tools to address an increasing number of applied questions and hypotheses. In order to use based detection techniques to best advantage, it is important to recognize only their advantages but also their limitations, such as the possibility particular NA-based tests may not have complete specificity for the of interest and only for that organism. The distinction between detection and disease diagnosis must also be recognized, and we believe NA-based tools are techniques for the former and not the latter. Several pathogen detection technologies are also discussed.
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a. identification of problem.